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Then Streptavidin-HRP is added and binds to the Biotinylated F8 antibody
The micro ELISA plate provided in this kit has been pre-coated with Rat Ang-â…¡
The concentration of Human WISP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat dLL4
which is also activated by PDGF
Human CERK (Ceramide Kinase) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human (CERK) Ceramide Kinase ELISA Kit measures Ceramide Kinase in samples. The plate has been pre coated with Human CERK antibody. CERK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CERK Antibody is added and binds to CERK in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CERK antibody. After incubation unbound Streptavidin HRP is washed away during a washing
