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Substrate solution is then added and color develops in proportion to the amount of Mouse CD9
And then biotinylated Mouse CDH11 Antibody is added and binds to CDH11 in the sample
And then biotinylated Mouse PLA2G10 Antibody is added and binds to PLA2G10 in the sample
MUC15 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF13
Canine F10A (Activated factor Xa heavy chain) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Canine F10A (Activated factor Xa heavy chain) ELISA Kit measures F10a in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti F10a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
