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Substrate solution is then added and color develops in proportion to the amount of Mouse HUWE1
NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity and affinity towards streptavidin
Calculate the concentration of FETUA in the sample by plotting standard curve
And then biotinylated Mouse EBI3 Antibody is added and binds to EBI3 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated QSOX1 antibody
Rat HPA (Heparanase) QuickTest ELISA Kit TSPAN30 Substrate solution is then addedThe Rat HPA (Heparanase) QuickTest ELISA Kit measures HPA Hpse in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual
