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it binds with Anti-CCL5 bound to precoated antigen
The plate has been pre-coated with Human SMURF1 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a2
Substrate solution is then added and color develops in proportion to the amount of Human KLC1
Calculate the concentration of Etaa1 in the sample by plotting standard curve
Rat SGP130 (soluble Glucoprotein 130) QuickTest ELISA Kit Packaging:96 wells it binds with Anti-CCL5 boundThe Rat SGP130 (soluble Glucoprotein 130) QuickTest ELISA Kit measures sgp130 in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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