Porcine IL-1b ELISA Kit Packaging:48 wells Mouse MT in the sample

Mouse MT in the sample or standard competes with a fixed amount of Mouse MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse MT

The gamma subunit is expressed primarily in neurons

Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat IL-1R2 are added to the micro ELISA plate wells

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL27A

Then a biotinylated detection antibody specific for Rat OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Porcine IL-1b ELISA Kit Packaging:48 wells Mouse MT in the sampleThe Porcine IL 1b ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Pig IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Pig IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those