Substrate solution is then added and color develops in proportion to the amount of Rat PPARA
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GCSF
And then biotinylated Mouse TCF7 Antibody is added and binds to TCF7 in the sample
Then a biotinylated detection antibody specific for Human IL-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated SLC14A1 antibody
Human CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human CYP1B1 (Cytochrome P450, family 1, subfamily B, polypeptide 1) ELISA Kit measures CYP1B1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CYP1B1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound