Human APLN (Apelin) ELISA Kit HH12 then added a HRP-conjugated antibody

then added a HRP-conjugated antibody specific to Mouse ADP

You can calculate the concentration of Human AFGF/FGF1 in the samples by comparing the OD of the samples to the standard curve

then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated

The OD value is proportional to the concentration of Human Aβ1-42

Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human APLN (Apelin) ELISA Kit HH12 then added a HRP-conjugated antibodyWith about 13 known citations, the Human APLN (Apelin) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human APLN. During the reaction, Human APLN in the sample or standard competes with a fixed amount of Human APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin