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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LBP
You can calculate the concentration of Human PCâ… in the samples by comparing the OD of the samples to the standard curve
The concentration of Human CCL14 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Rat MYH4 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated WNT8A antibody
Human WNT9A (Protein Wnt-9A) ELISA Kit ECGF Standards or samples are addedThe Human (WNT9A) Protein Wnt 9A ELISA Kit measures Protein Wnt 9A in samples. The plate has been pre coated with Human WNT9A antibody. WNT9A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT9A Antibody is added and binds to WNT9A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT9A antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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