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The OD value is proportional to the concentration of Human COL3
and the color intensity is directly proportional to the amount of Human VCAM1 present
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MIF
And then biotinylated Human CEACAM4 Antibody is added and binds to CEACAM4 in the sample
The assay plate has been pre-coated with mouse anti-Human IL-12 monoclonal antibody
Rat ITGA1 (Integrin Alpha-1) ELISA Kit Packaging:96 wells The OD value is proportionalThe Rat (ITGA1) Integrin Alpha 1 ELISA Kit measures Integrin Alpha 1 in Rat samples. The plate has been pre coated with Rat ITGA1 antibody. ITGA1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ITGA1 Antibody is added and binds to ITGA1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ITGA1 antibody. After incubation unbound Streptavidin HRP is washed away during a
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