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The concentration of Human HRH4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse DPP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PAX6
The plate has been pre-coated with Human APOD antibody
which are expressed on the surfaces of other cells
Rat NT-4 (Neurotrophin 4) ELISA Kit TRF The concentration of Human HRH4The Rat (NT 4) Neurotrophin 4 ELISA Kit measures Neurotrophin 4 in Rat samples. The plate has been pre coated with Rat NT 4 antibody. NT 4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 4 Antibody is added and binds to NT 4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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