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The concentration of Rat LRP5 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Bovine CHI3L1 Antibody is added and binds to CHI3L1 in the sample
only those wells that contain Human NFE2L2
ACY1 present in the sample is added and binds to antibodies coated on the wells
The Human (ASAH1) Acid Ceramidase ELISA Kit measures Acid Ceramidase in samples
Rat E2 (Estradiol) ELISA Kit TnTC The concentration of Rat LRP5The Rat (E2) Estradiol ELISA Kit measures Estradiol in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color develops.
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