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The concentration of Rat ACTH in tested samples can be calculated by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Canine IL5
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1a
The plate has been pre-coated with Human EMILIN1 antibody
Then a biotinylated detection antibody specific for Human GDF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human FGF7 (Fibroblast Growth Factor 7) ELISA Kit BPIFD The concentration of Rat ACTHThe Human (FGF7) Fibroblast Growth Factor 7 ELISA Kit measures Fibroblast Growth Factor 7 in samples. The plate has been pre coated with Human FGF7 antibody. FGF7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FGF7 Antibody is added and binds to FGF7 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FGF7 antibody. After incubation unbound Streptavidin HRP is washed away
