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Then Streptavidin-HRP is added and binds to the Biotinylated CSTA antibody
The concentration of IL1a in the samples is then determined by comparing the OD of the samples to the standard curve
FSTL1 present in the sample is added and binds to antibodies coated on the wells
a horseradish peroxidase conjugated mouse anti-Mouse S100A1 Antibody is added to the wells and binds to S100A1 in the sample
The Human TSP-2 (Thrombospondin-2) ELISA kit uses the Sandwich-ELISA principle
Porcine TLR4 (Toll-like Receptor 4) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Porcine (TLR4) Toll like Receptor 4 ELISA Kit measures Toll like receptor 4 in Porcine samples. The plate has been pre coated with Porcine TLR4 antibody. TLR4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine TLR4 Antibody is added and binds to TLR4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TLR4 antibody. After incubation unbound Streptavidin HRP is washed
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