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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgA are added to the micro ELISA plate wells
The assay plate has been pre-coated with mouse anti-Human c-MET monoclonal antibody
a horseradish peroxidase conjugated mouse anti-Human Factor H Antibody is added to the wells and binds to Factor H in the sample
only those wells that contain Human PLD
The OD value is proportional to the concentration of Rat ApoH
Rat IL6 (Interleukin 6) ELISA Kit (Small Sample) Packaging:48 wells Samples (or Standards) and HorseradishThe Rat IL6 (Interleukin 6) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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