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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF
Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Unbound avidin-HRP is washed away during a washing step
Alpha-Actinin is necessary for the attachment of actin filaments to the Z-lines in skeletal muscle cells
Substrate solution is then added and color develops in proportion to the amount of Mouse IL24
Rat SE-P (Selenoprotein-P) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (SE P) Selenoprotein P ELISA Kit measures Selenoprotein P in Rat samples. The plate has been pre coated with Rat Se P antibody. Se P present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Se P Antibody is added and binds to Se P in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Se P antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
