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Substrate solution is then added and color develops in proportion to the amount of Human CYTIP
and the color intensity is directly proportional to the amount of Human FOLR1 present
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSC22
The concentration of Guinea pig IL1a in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Rat BHMT
Human IL-2R (Interleukin-2 Receptor) ELISA Kit CLG4 Substrate solution is then addedThe Human (IL 2R) Interleukin 2 Receptor ELISA Kit measures Interleukin 2 Receptor in samples. The plate has been pre coated with Human IL2R antibody. IL2R present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL2R Antibody is added and binds to IL2R in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IL2R antibody. After incubation unbound Streptavidin HRP is washed away during
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