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Then a biotinylated detection antibody specific for Human SOD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
absorbance at 450nm in a microplate reader
ICAM3 present in the sample is added and binds to antibodies coated on the wells
The Human IDS (Iduronate 2-sulfatase) ELISA Kit measures IDS in Human Serum
it binds with MIP4α bound to precoated antibody
Human NANOG (Homeobox protein NANOG) QuickTest ELISA Kit CD284 Then a biotinylated detection antibodyThe Human NANOG (Homeobox protein NANOG) QuickTest ELISA Kit measures NANOG in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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