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You can calculate the concentration of Human TGF-Beta in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rat FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
the Rat EPO (Erythropoietin) ELISA kit uses the Sandwich-ELISA principle
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Only those wells that contain Mouse TNF-alpha
CD20 Mouse mAb PGHS-2 You can calculate the concentrationCD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes.