Human CD248 (Endosialin) ELISA Kit CGL1 The micro ELISA plate provided

The micro ELISA plate provided in this kit has been pre-coated with Sheep E3

Only those wells that contain Human bFGF/FGF2

The assay plate has been pre-coated with mouse anti-Human CEACAM6 monoclonal antibody

Then a biotinylated detection antibody specific for Rat IgM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Substrate solution is then added and color develops in proportion to the amount of Human CBX1

Human CD248 (Endosialin) ELISA Kit CGL1 The micro ELISA plate providedThe Human (CD248) Endosialin ELISA Kit measures Endosialin in samples. The plate has been pre coated with Human CD248 antibody. CD248 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD248 Antibody is added and binds to CD248 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CD248 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.