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Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
CYP27A1 present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Human NFKB-p65
GAPDH is constitutively expressed in almost all tissues at high levels
The concentration of Human CRHR2 in the samples is then determined by comparing the OD of the samples to the standard curve
Human CPNE9 (Copine 9) ELISA Kit CRP Then a biotinylated detection antibodyThe Human (CPNE9) Copine 9 ELISA Kit measures Copine 9 in samples. The plate has been pre coated with Human CPNE9 antibody. CPNE9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CPNE9 Antibody is added and binds to CPNE9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CPNE9 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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