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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 15 nm
a capture antibody-SRPX2-biotin-detection antibody complex was formed
The plate has been pre-coated with Rabbit Trx antibody
The concentration of Human ELA3A in the samples is then determined by comparing the OD of the samples to the standard curve
The Equine Leptin ELISA kit is a sandwich enzyme immunoassay
Human CENPB (Centromere Protein B) ELISA Kit P3NP The optical density (OD) isThe Human CENPB (Centromere Protein B) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human CENPB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CENPB and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro
