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Then Streptavidin-HRP is added and binds to the Biotinylated EXOSC4 antibody
Then a biotinylated detection antibody specific for Human SPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated IL17A antibody
Nmur1 present in the sample is added and binds to antibodies coated on the wells
The OD value is proportional to the concentration of Rat MMP-9
Rat GPX-1 (Glutathione Peroxidase 1) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat (GPX 1) Glutathione Peroxidase 1 ELISA Kit measures Glutathione Peroxidase 1 in Rat samples. The plate has been pre coated with Rat GPX1 antibody. GPX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GPX1 Antibody is added and binds to GPX1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GPX1 antibody. After incubation unbound Streptavidin HRP is washed away
