Rat IFN gamma (Interferon Gamma) ELISA Kit PCT The concentration of Human Irisin

The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Mouse IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Recombinant Human Vascular Endothelial Growth Factor D is produced by our Mammalian expression system and the target gene encoding Phe93-Ser201 is expressed with a 6His tag at the C-terminus

Only those wells that contain Rat TSH

Rat IFN gamma (Interferon Gamma) ELISA Kit PCT The concentration of Human IrisinThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IFN Gamma. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IFN Gamma. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is