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a horseradish peroxidase conjugated mouse anti-Human B4GALT1 Antibody is added to the wells and binds to B4GALT1 in the sample
Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human PAP
The concentration of CCL16 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat KIM-1 (Kidney Injury Molecule 1) ELISA Kit N a horseradish peroxidase conjugated mouseThis Rat KIM 1 (Kidney Injury Molecule 1) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat KIM 1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat KIM 1 are added to the micro ELISA plate wells. Rat KIM 1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat KIM 1.
