Human C5A (Complement Component 5a) ELISA Kit PBSF Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated PHF7 antibody

The concentration of Human GP5 in the samples is then determined by comparing the OD of the samples to the standard curve

The Human EDN (Eosinophil-Derived Neurotoxin) ELISA kit uses the Sandwich-ELISA principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A8

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 43 nm

Human C5A (Complement Component 5a) ELISA Kit PBSF Then Streptavidin-HRP is added andThe Human C5A (Complement Component 5a) ELISA Kit measures C5a in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C5a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection