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Substrate solution is then added and color develops in proportion to the amount of Human SMAD2
Then Streptavidin-HRP is added and binds to the Biotinylated CXADR antibody
The microplate provided in this kit has been precoated with anti pro-BDNF antibody
SNX9 present in the sample is added and binds to antibodies coated on the wells
The concentration of HNP1-3 in the sample was calculated by drawing a standard curve
Human RF-IgA (Rheumatoid Factor IgA) ELISA Kit Hypocretin- Substrate solution is then addedThe Human RF IgA (Rheumatoid Factor IgA) ELISA Kit measures RF IgA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
