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GDF10 present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Mouse CTSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human ALOX5AP Antibody is added and binds to ALOX5AP in the sample
The microplate provided in this kit has been precoated with anti SIRT1 antibody
And then biotinylated Human PSAPL1 Antibody is added and binds to PSAPL1 in the sample
Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA Kit Packaging:96 wells GDF10 present in the sampleThe Rat VIP (Vasoactive Intestinal Peptide) QuickTest ELISA Kit measures VIP in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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