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Substrate solution is then added and color develops in proportion to the amount of Human SLC25A12
If the sample contains IL-8
If the sample contains GAL14
And then biotinylated Rat AQP2 Antibody is added and binds to AQP2 in the sample
The plate has been pre-coated with Human STX18 antibody
Guinea pig AP13 (Apelin 13) ELISA Kit Packaging:48 wells Substrate solution is then addedThe Guinea pig AP13 (Apelin 13) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Guinea pig AP13. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Guinea pig AP13. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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